Well, you asked so here goes....
We've been running Vista since November (Business Edition {Final - NOT Beta} was made available to customers with MVL's {Multi Volume License} back in November). Of course most of the drivers are only now comming out for hardware, so it was tough going in the begining. But it's nice to work for a MS gold partner, and work on a customer's site who has a MVL...you tend to get access to stuff quite quickly 
I like it, despite a few anoying "features", mainly in the so called high security features. It is these features which actualy breaks some (a lot actualy) old software, thus why some apps like Limewire don't run so good on Vista.
You need good hardware to run it on, if you want to take full advantage of it, but overall you can turn a lot of features off. We haven't had many problems, besides the intial few incompatibilities and it's been fun putting it through it paces. We've run it on Dell desktops (P4 3Ghz, 1Gb Ram) and laptops (similar spec, just the M and Core 2 Duo processors). Lots of nice things, takes some getting used to the new interface, but it grows on you quite fast, and if you've ever used linux, then you will find your way very quickly.
I did get it to run on a P3 with 384Mb ram, had to install 512Mb (otherwise the installer won't install), then take it back to 384Mb. Ran suprizingly well, provided you don't try to do too much, or expect too much 
Overall, IMHO, it is quite nice, and will take time for large businesses to take it seriously, due to the hardware requirements, but it is the same process we went through with XP.
I use it on my work PC, and so far I'm happy...and believe me, that machine gets worked...
One VERY nasty thing we did find...if you turn on the indexing for the very nice search features, and you are running Office 2007 as well on the machine, your hard drive space disappears very rapidly.
Another nasty, is if your PC is on a domain (as in MS networking domain, not internet domain name), then the damn thing takes forever and a day to log in the first time, and every now and then again afterwards.
Office 2007 (yes we've been running that for a while as well), is much better than the beta releases, and I simply love it. If you use it for a few days then go back to a machine with office 2003 on, it's a horrible feeling...!
Only thing I'm not looking forward to, is the MS Vista exams, which I will stall until late this year or maybe even next year...when they've made up their minds how it's going to fit in with the grand scheme of things and there's more material around (they're busy with the beta versions of the MS Vista exams, if not just finished by now )..
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